BCL3 antibody (AA 127-376)

Catalog No. ABIN1858125

This anti-BCL3 antibody is a Rabbit Polyclonal antibody detecting BCL3 in WB, IHC, IP and ICC. Suitable for Rat.

Quick Overview for BCL3 antibody (AA 127-376) (ABIN1858125)

Target: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-BCL3 Antibody

Binding Specificity: AA 127-376

Purpose: Polyclonal Antibody to B-Cell CLL/Lymphoma 3 (Bcl3)

Specificity: The antibody is a rabbit polyclonal antibody raised against Bcl3. It has been selected for its ability to recognize Bcl3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Bcl3 (Asp127-Asn376)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BCL3

Target: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Alternative Name: Bcl3

Background: BCL3, BCL4, B-Cell Lymphoma 3-Encoded Protein, B-Cell Lymphoma 3-Encoded Protein, Chronic Lymphatic Leukemia Protein, Proto-oncogene BCL3

Pathways: NF-kappaB Signaling, Maintenance of Protein Location, Protein targeting to Nucleus