MIF antibody (AA 4-112)

Catalog No. ABIN1859825

Product Details anti-MIF Antibody

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Binding Specificity: AA 4-112

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against MIF. It has been selected for its ability to recognize MIF in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: MIF (Phe4-Ser112)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

References for anti-MIF antibody (ABIN1859825)

Eldomiaty, Elayat, Ali, Algaidi, Elnaggar: "Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines' expression." in: Folia morphologica, Vol. 79, Issue 2, pp. 350-358, (2020) (PubMed).

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: Macrophage Migration Inhibitory Factor (MIF) (MIF Products)

Synonyms: mif antibody, Mif antibody, gif antibody, glif antibody, mmif antibody, LOC100136498 antibody, LOC100284350 antibody, LOC100284546 antibody, GIF antibody, Glif antibody, GLIF antibody, MMIF antibody, macrophage migration inhibitory factor L homeolog antibody, macrophage migration inhibitory factor antibody, macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody, mif.L antibody, mif antibody, MIF antibody, Mif antibody, PHATRDRAFT_49660 antibody, LOC100136498 antibody, cl405_1 antibody, LOC100284546 antibody

Background: Alternative Names: GIF, GLIF, MMIF, Glycosylation-Inhibiting Factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling