Nicastrin antibody (AA 420-655)

Catalog No. ABIN1859974

This Rabbit Polyclonal antibody specifically detects Nicastrin in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Nicastrin antibody (AA 420-655) (ABIN1859974)

Target: Nicastrin (NCSTN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicastrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Nicastrin Antibody

Binding Specificity: AA 420-655

Purpose: Polyclonal Antibody to Nicastrin (NCSTN)

Specificity: The antibody is a rabbit polyclonal antibody raised against NCSTN. It has been selected for its ability to recognize NCSTN in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: NCSTN (Gln420-Asp655)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nicastrin

Target: Nicastrin (NCSTN)

Alternative Name: NCSTN

Background: APH2, NCT

Pathways: Notch Signaling, Neurotrophin Signaling Pathway