PRAME antibody (AA 416-502)

Catalog No. ABIN1860297

Product Details anti-PRAME Antibody

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Binding Specificity: AA 416-502

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAME antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against PRAME. It has been selected for its ability to recognize PRAME in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: PRAME (Asn416-Leu502)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Abstract: PRAME Products

Synonyms: PRAME antibody, CT130 antibody, MAPE antibody, OIP-4 antibody, OIP4 antibody, 4930534P07Rik antibody, preferentially expressed antigen in melanoma antibody, PRAME antibody, Prame antibody

Background: Alternative Names: MAPE, OIP4, CT130, Cancer/Testis Antigen 130, Opa-interacting protein 4, Melanoma antigen preferentially expressed in tumors

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding