SP100 antibody (AA 261-465)

Catalog No. ABIN1860601

Product Details anti-SP100 Antibody

Target: SP100 (SP100 Nuclear Antigen (SP100))

Binding Specificity: AA 261-465

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP100 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Sp100 Nuclear Antigen (Sp100)

Specificity: The antibody is a rabbit polyclonal antibody raised against Sp100. It has been selected for its ability to recognize Sp100 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA326Mu01Recombinant Sp100 Nuclear Antigen (Sp100)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SP100

Target: SP100 (SP100 Nuclear Antigen (SP100))

Alternative Name: Sp100 (SP100 Products)

Background: Speckled 100 kDa, Nuclear dot-associated Sp100 protein

Pathways: Retinoic Acid Receptor Signaling Pathway