ITK antibody (AA 368-625)

Catalog No. ABIN1868785

This Rabbit Polyclonal antibody specifically detects ITK in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for ITK antibody (AA 368-625) (ABIN1868785)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ITK Antibody

Binding Specificity: AA 368-625

Purpose: Polyclonal Antibody to IL2 Inducible T-Cell Kinase (ITK)

Specificity: The antibody is a rabbit polyclonal antibody raised against ITK. It has been selected for its ability to recognize ITK in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant IL2 Inducible T-Cell Kinase (ITK) corresdonding to Leu368~Leu625 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: IL2 Inducible T-Cell Kinase

Background: PSCTK2, EMT, LYK, T-cell-specific kinase, Tyrosine-protein kinase Lyk

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway