LAT antibody (AA 25-221)

Catalog No. ABIN1868912

The Rabbit Polyclonal anti-LAT antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect LAT in samples from Human.

Quick Overview for LAT antibody (AA 25-221) (ABIN1868912)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-LAT Antibody

Binding Specificity: AA 25-221

Purpose: Polyclonal Antibody to Linker For Activation Of T-Cell (LAT)

Specificity: The antibody is a rabbit polyclonal antibody raised against LAT. It has been selected for its ability to recognize LAT in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA270Hu01Recombinant Linker For Activation Of TCell (LAT)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: Linker For Activation Of T-Cell

Background: LAT1, Pp36, Linker Of Activated T Cells, Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway