MCL-1 antibody (AA 6-328)

Catalog No. ABIN1869117

Cited in 1 publication. The Rabbit Polyclonal anti-MCL-1 antibody (ABIN1869117) specifically detects MCL-1 in WB, IHC, ICC and IP. The antibody is reactive with Human samples.

Quick Overview for MCL-1 antibody (AA 6-328) (ABIN1869117)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-MCL-1 Antibody

Binding Specificity: AA 6-328

Purpose: Polyclonal Antibody to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)

Specificity: The antibody is a rabbit polyclonal antibody raised against MCL1. It has been selected for its ability to recognize MCL1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) corresdonding to Arg6~Ile328 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-MCL-1 antibody (ABIN1869117)

Bharambe, Joshi, Yogi, Kazi, Shirsat: "Restoration of miR-193a expression is tumor-suppressive in MYC amplified Group 3 medulloblastoma." in: Acta neuropathologica communications, Vol. 8, Issue 1, pp. 70, (2020) (PubMed).

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL1

Background: EAT, MCL1L, MCL1S, BCL2L3, TM, Myeloid Cell Leukemia Sequence 1, Bcl2 Related, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT

Pathways: MAPK Signaling