TIE1 antibody (AA 835-1134)

Catalog No. ABIN1871557

This Rabbit Polyclonal antibody specifically detects TIE1 in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for TIE1 antibody (AA 835-1134) (ABIN1871557)

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-TIE1 Antibody

Binding Specificity: AA 835-1134

Purpose: Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

Specificity: The antibody is a rabbit polyclonal antibody raised against Tie1. It has been selected for its ability to recognize Tie1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA125Mu01Recombinant Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: Tie1

Background: JTK14, Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1, Tyrosine-protein kinase receptor Tie-1

Pathways: RTK Signaling