Caspase 3 antibody (N-Term)

Catalog No. ABIN187517

The Mouse Monoclonal anti-Caspase 3 antibody is suitable to detect Caspase 3 in samples from Human. It has been validated for WB.

Quick Overview for Caspase 3 antibody (N-Term) (ABIN187517)

Target: Caspase 3 (CASP3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: AM1-4

Product Details anti-Caspase 3 Antibody

Binding Specificity: N-Term

Specificity: Species Reactivity: Human, others not tested.

Sterility: 0.2 μm filtered

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human Caspase-3 protein and mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: Western Blot The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Mouse monoclonal antibody against human Caspase-3 (cysteine-requiring aspartate protease-3). Available in 100 ul vials at a concentration of 1 mg/ml (100ug) in PBS with 0.08% sodium azide. Purified and 0.2 µm sterile filtered.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: Caspase-3 along with caspase 7 and 6 form a group of effector caspases that are responsible for the cleavage of multiple substrates including the cytyokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 occurs in three variant forms. Caspase-3-like activities are required for Fas-mediated apoptosis. However, the role of caspase-1 and caspase-3 in mediating Fas-induced cell death is not clear. Although wild-type, caspase-1(-/-), and caspase-3(-/-) hepatocytes were killed at a similar rate when co-cultured with FasL expressing NIH 3T3 cells, caspase-3(-/-) hepatocytes displayed drastically different morphological changes as well as significantly delayed DNA fragmentation observed in hepatocytes. Cleavage of various caspase substrates implicates apoptotic events, including gesolin, fodin, lamin B, and DFF45/ICAD are delayed or absent. The altered cleavage of these key substrates is likely responsible for the aberrant apoptosis observed in both hepatocytes and thymocytes deficient in caspase-3.

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation