LEF1 antibody

Catalog No. ABIN187758

The Sheep Polyclonal anti-LEF1 antibody (ABIN187758) specifically detects LEF1 in WB. The antibody is reactive with Human samples.

Quick Overview for LEF1 antibody (ABIN187758)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LEF1 Antibody

Specificity: Antibody reacts with human LEF-1, TCF-1, TCF-3, and TCF-4 Species Reactivity: Human, other species not tested.

Immunogen: Full length LEF

Application Details

Application Notes: Western blot: Use at 1-5 µg/ml The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 mg/ml purified IgG in PBS with 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background: Lymphoid Enhancer Factor - 1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3, and TCF-4) These factors play a crucial role in WNT/Wingless signaling, a transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also involved in the genesis of some cancers, including colon cancer, melanoma, and breast cancer. LEF-1 is expressed during development in many differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers, and the trigeminal nerve. It is redundant with TCF-1 (T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE gels. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They may appear on Western blots as smaller immunoreactive forms.

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding