CHEK1 antibody (AA 1-474)

Catalog No. ABIN187834

This Sheep Polyclonal antibody specifically detects CHEK1 in WB. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for CHEK1 antibody (AA 1-474) (ABIN187834)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Sheep

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 1-474

Specificity: Recognizes chk1 kinase. Species Reactivity: Human, monkey, mouse, and rat, other species not tested. Molecular Weight: .54 kDa. Cellular Location: Cytoplasmic.

Sterility: 0.2 μm filtered

Immunogen: GST fusion protein corresponding to amino acid sequence 1-474 of human chk1 kinase.

Application Details

Application Notes: Western blot: Use 1-10 µg/ml. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Provided as 0.2 µm sterile filtered solution in PBS with 0.08% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1

Background: One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair