ADAM17 antibody (C-Term)

Catalog No. ABIN187860

The Rabbit Polyclonal anti-ADAM17 antibody (ABIN187860) specifically detects ADAM17 in WB. The antibody is reactive with Human samples.

Quick Overview for ADAM17 antibody (C-Term) (ABIN187860)

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADAM17 Antibody

Binding Specificity: C-Term

Specificity: Detects TACE by Western blot at 0.25 to 1 µg/ml. Detects an approximately 130 kDa band in HeLa and Jurkat whole cell lysate which may represent mature protein, precursor, and glycosylated TACE. Species Reactivity: Human, other species not tested. Positive Control: HeLa and Jurkat whole cell lysates.

Immunogen: Synthetic peptide corresponding to amino acids 807 to 823 of the human TACE protein. Immunogen sequence differs from the mouse and rat sequences by one amino acid.

Application Details

Application Notes: Western blot The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: Purified antibody provided in PBS solution containing 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: CD156b / ADAM17

Background: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintergrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus