p300 antibody (pSer1834)

Catalog No. ABIN1881299

The Rabbit Polyclonal anti-p300 antibody has been validated for DB. It is suitable to detect p300 in samples from Human. There are 5+ publications available.

Quick Overview for p300 antibody (pSer1834) (ABIN1881299)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is un-conjugated

Application: Dot Blot (DB)

Clone: RB41431

Product Details anti-p300 Antibody

Binding Specificity: pSer1834

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This EP300 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1834 of human EP300.

Isotype: Ig Fraction

Application Details

Application Notes: DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-p300 antibody (ABIN1881299)

Zhang, Zhang, Rui, Liu: "p300-mediated acetylation stabilizes the Th-inducing POK factor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 3960-9, (2010) (PubMed).

Vempati, Jayani, Notani, Sengupta, Galande, Haldar: "p300-mediated acetylation of histone H3 lysine 56 functions in DNA damage response in mammals." in: The Journal of biological chemistry, Vol. 285, Issue 37, pp. 28553-64, (2010) (PubMed).

Reynoird, Schwartz, Delvecchio, Sadoul, Meyers, Mukherjee, Caron, Kimura, Rousseaux, Cole, Panne, French, Khochbin: "Oncogenesis by sequestration of CBP/p300 in transcriptionally inactive hyperacetylated chromatin domains." in: The EMBO journal, Vol. 29, Issue 17, pp. 2943-52, (2010) (PubMed).

Jang, Choi, Jeong, Lee: "Phosphorylation of p300 by ATM controls the stability of NBS1." in: Biochemical and biophysical research communications, Vol. 397, Issue 4, pp. 637-43, (2010) (PubMed).

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, (2010) (PubMed).

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300

Background: This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.

Molecular Weight: 264161

NCBI Accession: NP_001420

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size