p53 antibody (pSer9)

Catalog No. ABIN2850450

This anti-p53 antibody is a Rabbit Polyclonal antibody detecting p53 in WB, IF and DB. Suitable for Human. This Primary Antibody has been cited in 5+ publications.

Quick Overview for p53 antibody (pSer9) (ABIN2850450)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Clone: RB7893

Product Details anti-p53 Antibody

Binding Specificity: pSer9

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This P53 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S9 of human P53.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-p53 antibody (ABIN2850450)

Blanchette, Cheng, Yan, Ketner, Ornelles, Dobner, Conaway, Conaway, Branton: "Both BC-box motifs of adenovirus protein E4orf6 are required to efficiently assemble an E3 ligase complex that degrades p53." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9619-29, (2004) (PubMed).

Adachi, Toyota, Sasaki, Yamashita, Ishida, Ohe-Toyota, Maruyama, Hinoda, Saito, Imai, Kudo, Tokino: "Identification of SCN3B as a novel p53-inducible proapoptotic gene." in: Oncogene, Vol. 23, Issue 47, pp. 7791-8, (2004) (PubMed).

Zhang, Wang, Chen, Zhang, Cheng, Heng, Wu, Shen: "Repression of hsp90beta gene by p53 in UV irradiation-induced apoptosis of Jurkat cells." in: The Journal of biological chemistry, Vol. 279, Issue 41, pp. 42545-51, (2004) (PubMed).

Anazawa, Arakawa, Nakagawa, Nakamura: "Identification of STAG1 as a key mediator of a p53-dependent apoptotic pathway." in: Oncogene, Vol. 23, Issue 46, pp. 7621-7, (2004) (PubMed).

Montagnoli, Tenca, Sola, Carpani, Brotherton, Albanese, Santocanale: "Cdc7 inhibition reveals a p53-dependent replication checkpoint that is defective in cancer cells." in: Cancer research, Vol. 64, Issue 19, pp. 7110-6, (2004) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: P53

Background: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.

Molecular Weight: 43653

NCBI Accession: NP_000537, NP_001119584, NP_001119585, NP_001119586, NP_001119587, NP_001119588, NP_001119589, NP_001119590, NP_001263624, NP_001263625, NP_001263626, NP_001263627, NP_001263628, NP_00126

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect