IKBKB antibody

Catalog No. ABIN1882097

There are 8+ publications for this product available. The Rabbit Polyclonal anti-IKBKB antibody is suitable to detect IKBKB in samples from Human. It has been validated for WB, IF and IHC (p).

Quick Overview for IKBKB antibody (ABIN1882097)

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKBKB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB2161

Product Details anti-IKBKB Antibody

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This IKK beta antibody is generated from rabbits immunized with recombinant human IKK beta (full length sequence).

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-IKBKB antibody (ABIN1882097)

Le Henaff, Mansouri, Modrowski, Zarka, Geoffroy, Marty, Tarantino, Laplantine, Marie: "Increased NF-?B Activity and Decreased Wnt/?-Catenin Signaling Mediate Reduced Osteoblast Differentiation and Function in ?F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Mice." in: The Journal of biological chemistry, Vol. 290, Issue 29, pp. 18009-17, (2015) (PubMed).

Gouin, Adib-Conquy, Balestrino, Nahori, Villiers, Colland, Dramsi, Dussurget, Cossart: "The Listeria monocytogenes InlC protein interferes with innate immune responses by targeting the I{kappa}B kinase subunit IKK{alpha}." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 40, pp. 17333-8, (2010) (PubMed).

Higashimoto, Chan, Lee, Zandi: "Regulation of I(kappa)B kinase complex by phosphorylation of (gamma)-binding domain of I(kappa)B kinase (beta) by Polo-like kinase 1." in: The Journal of biological chemistry, Vol. 283, Issue 51, pp. 35354-67, (2008) (PubMed).

Tang, Inohara, Wang, Nuñez, Guan: "Roles for homotypic interactions and transautophosphorylation in IkappaB kinase beta IKKbeta) activation [corrected]." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38566-70, (2003) (PubMed).

Ebner, Bandion, Binder, de Martin, Schmid: "GMCSF activates NF-kappaB via direct interaction of the GMCSF receptor with IkappaB kinase beta." in: Blood, Vol. 102, Issue 1, pp. 192-9, (2003) (PubMed).

Carter, Pennington, Ungurait, Ballard: "In vivo identification of inducible phosphoacceptors in the IKKgamma/NEMO subunit of human IkappaB kinase." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19642-8, (2003) (PubMed).

Huang, Chen, Chen: "c-Src-dependent tyrosine phosphorylation of IKKbeta is involved in tumor necrosis factor-alpha-induced intercellular adhesion molecule-1 expression." in: The Journal of biological chemistry, Vol. 278, Issue 11, pp. 9944-52, (2003) (PubMed).

Sakurai, Suzuki, Kawasaki, Nakano, Okazaki, Chino, Doi, Saiki: "Tumor necrosis factor-alpha-induced IKK phosphorylation of NF-kappaB p65 on serine 536 is mediated through the TRAF2, TRAF5, and TAK1 signaling pathway." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36916-23, (2003) (PubMed).

Target Details for IKBKB

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Alternative Name: IKK beta

Background: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Molecular Weight: 86564

NCBI Accession: NP_001177649, NP_001229707, NP_001547

UniProt: O14920

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins