TYMS antibody (C-Term)

Catalog No. ABIN1882144

This Rabbit Polyclonal antibody specifically detects TYMS in WB, IF, FACS and IHC (p). It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for TYMS antibody (C-Term) (ABIN1882144)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB20041

Product Details anti-TYMS Antibody

Binding Specificity: AA 265-294, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This TYSY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human TYSY.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-TYMS antibody (ABIN1882144)

Ma, He, Zhang, Fei, Niu, Wang, Ding, Xu, Chen, Zhu: "DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5639-49, (2012) (PubMed).

Ren, Kim, Koh, Chang, Eom, Yi, Seong, Kim, Bronner, Cho: "Comparative analysis of thymidylate synthase at the protein, mRNA, and DNA levels as prognostic markers in colorectal adenocarcinoma." in: Journal of surgical oncology, Vol. 100, Issue 7, pp. 546-52, (2009) (PubMed).

Schiffer, Clifton, Davisson, Santi, Stroud: "Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site." in: Biochemistry, Vol. 34, Issue 50, pp. 16279-87, (1996) (PubMed).

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Background: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.

Molecular Weight: 35716

NCBI Accession: NP_001062

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases