ATG3 antibody (C-Term)

Catalog No. ABIN1882157

The Rabbit Polyclonal anti-ATG3 antibody has been validated for WB. It is suitable to detect ATG3 in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for ATG3 antibody (C-Term) (ABIN1882157)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB7459

Product Details anti-ATG3 Antibody

Binding Specificity: AA 168-197, C-Term

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the C-terminal region of human ATG3.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ATG3 antibody (ABIN1882157)

Radoshevich, Murrow, Chen, Fernandez, Roy, Fung, Debnath: "ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and cell death." in: Cell, Vol. 142, Issue 4, pp. 590-600, (2010) (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, (2004) (PubMed).

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: ATG3

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Molecular Weight: 35864

NCBI Accession: NP_001265641, NP_071933

UniProt: Q9NT62

Pathways: Autophagy