Aurora A antibody (C-Term)

Catalog No. ABIN1882163

The Rabbit Polyclonal anti-Aurora A antibody has been validated for WB, IF and FACS. It is suitable to detect Aurora A in samples from Human. There are 6+ publications available.

Quick Overview for Aurora A antibody (C-Term) (ABIN1882163)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RB6834

Product Details anti-Aurora A Antibody

Binding Specificity: AA 364-392, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Aurora-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human Aurora-A.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:1000. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-Aurora A antibody (ABIN1882163)

Nakamura, Hirano, Okinaka, Takemura, Yokota, Ono, Shigeno, Shibata, Fujisawa, Ohnishi: "The FOXM1 transcriptional factor promotes the proliferation of leukemia cells through modulation of cell cycle progression in acute myeloid leukemia." in: Carcinogenesis, Vol. 31, Issue 11, pp. 2012-21, (2010) (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, (2002) (PubMed).

Tanaka, Ueda, Kanamori, Ideguchi, Yang, Kitajima, Ishigatsubo: "Cell-cycle-dependent regulation of human aurora A transcription is mediated by periodic repression of E4TF1." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10719-26, (2002) (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

Nigg: "Mitotic kinases as regulators of cell division and its checkpoints." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 1, pp. 21-32, (2001) (PubMed).

Shindo, Nakano, Kuroyanagi, Shirasawa, Mihara, Gilbert, Jenkins, Copeland, Yagita, Okumura: "cDNA cloning, expression, subcellular localization, and chromosomal assignment of mammalian aurora homologues, aurora-related kinase (ARK) 1 and 2." in: Biochemical and biophysical research communications, Vol. 244, Issue 1, pp. 285-92, (1998) (PubMed).

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: Aurora-A

Background: Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Auroria kinase A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It may be involved in microtubule formation and/or stabilization. This protein has also been postulated to play a key role during tumor development and progression. Aurora kinase A localizes on centrosomes in interphase cells and at each spindle pole in mitosis. It is highly expressed in testis, weakly in skeletal muscle, thymus and spleen, and also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly afterward. Defects in Aurora kinase A are responsible for numerical centrosome aberrations including aneuploidy.

Molecular Weight: 45809

NCBI Accession: NP_003591, NP_940835, NP_940836, NP_940837, NP_940838, NP_940839

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization