LEF1 antibody (AA 10-37)

Catalog No. ABIN1882259

Product Details anti-LEF1 Antibody

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Binding Specificity: AA 10-37

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: M, Rat

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogen: This LEF1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 10-37 amino acids from human LEF1.

Clone: 661CT8-3-5

Isotype: IgG1

Application Details

Application Notes: WB: 1:500~8000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-LEF1 antibody (ABIN1882259)

Gutierrez, Tschumper, Wu, Shanafelt, Eckel-Passow, Huddleston, Slager, Kay, Jelinek: "LEF-1 is a prosurvival factor in chronic lymphocytic leukemia and is expressed in the preleukemic state of monoclonal B-cell lymphocytosis." in: Blood, Vol. 116, Issue 16, pp. 2975-83, (2010) (PubMed).

Kalsi, Kuo, Aliev, Alexander, McMichael, Patterson, Walsh, Zhao, Schuckit, Nurnberger, Edenberg, Kramer, Hesselbrock, Tischfield, Vladimirov, Prescott, Dick, Kendler, Riley: "A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts." in: Human molecular genetics, Vol. 19, Issue 12, pp. 2497-506, (2010) (PubMed).

Chen, Zhang, Pincus, Wu, Ricks, Liu, Sun, Maclaren, Lan: "Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 5047-54, (2010) (PubMed).

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1 (LEF1 Products)

Background: This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Molecular Weight: 44201

NCBI Accession: NP_001124185, NP_001124186, NP_001159591, NP_057353

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding