RAG2 antibody (AA 271-519)

Catalog No. ABIN1886297

The Rabbit Polyclonal anti-RAG2 antibody is suitable to detect RAG2 in samples from Human. It has been validated for WB, IHC and IF.

Quick Overview for RAG2 antibody (AA 271-519) (ABIN1886297)

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RAG2 Antibody

Binding Specificity: AA 271-519

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 271 and 519 of RAG2

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.100-1.500
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Alternative Name: RAG2

Background: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development.This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences.The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA.A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4.Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.[provided by RefSeq]

Molecular Weight: 59 kDa

Gene ID: 5897

NCBI Accession: NP_000527, NM_000536

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response