LAMC2 antibody (AA 445-627)

Catalog No. ABIN1886477

The Rabbit Polyclonal anti-LAMC2 antibody has been validated for WB and IHC. It is suitable to detect LAMC2 in samples from Human.

Quick Overview for LAMC2 antibody (AA 445-627) (ABIN1886477)

Target: LAMC2 (Laminin, gamma 2 (LAMC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-LAMC2 Antibody

Binding Specificity: AA 445-627

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 445 and 627 of Human LAMC2

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.100-1.250

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 20 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for LAMC2

Target: LAMC2 (Laminin, gamma 2 (LAMC2))

Alternative Name: Laminin gamma-2

Background: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes.They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis.Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains.Each laminin chain is a multidomain protein encoded by a distinct gene.Several isoforms of each chain have been described.Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e.alpha1beta1gamma1 heterotrimer is laminin 1.The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo.This gene encodes the gamma chain isoform laminin, gamma 2.The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain, however, it lacks domain VI, and domains V, IV and III are shorter.It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney.The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane.The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction.Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described.The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known.Transcript variants utilizing alternative polyA_signal have also been noted in literature.[provided by RefSeq]

Molecular Weight: 122 kDa

Gene ID: 3918