IDE antibody (Internal Region)

Catalog No. ABIN190754

The Goat Polyclonal anti-IDE antibody is suitable to detect IDE in samples from Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for IDE antibody (Internal Region) (ABIN190754)

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IDE antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IDE Antibody

Binding Specificity: Internal Region

Purpose: Ide (mouse)

Sequence: QQYNYDRDNI E

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QQYNYDRDNIE, from the internal region of the protein sequence according to NP_112419.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118 kDa according to NP_112419.2). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. This

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: Ide

Background: Ide, insulin-degrading enzyme, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSULYSIN, insulinase, insulysin

Gene ID: 15925, 25700

NCBI Accession: NP_112419

Pathways: SARS-CoV-2 Protein Interactome