CD11b antibody (Internal Region)

Catalog No. ABIN190775

The Goat Polyclonal anti-CD11b antibody has been validated for IHC, IF and ELISA. It is suitable to detect CD11b in samples from Human.

Quick Overview for CD11b antibody (Internal Region) (ABIN190775)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Grade: Verified

Product Details anti-CD11b Antibody

Binding Specificity: Internal Region

Purpose: ITGAM / CD11B

Sequence: RNDGEDSYRT Q

Specificity: The immunizing peptide represents part of the extra-cellular domain.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RNDGEDSYRTQ, from the internal region of the protein sequence according to NP_000623.2 .

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 6-8 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of U937 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: ITGAM

Background: ITGAM , integrin, alpha M (complement component 3 receptor 3 subunit) , CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A , Integrin, alpha-M (complement component receptor-3, alpha), antigen CD11B (p170), macrophage antigen, alpha polypeptide, antigen CD11b (p

Gene ID: 3684, 16409, 25021

NCBI Accession: NP_000623

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation