ILF2 antibody (Internal Region)

Catalog No. ABIN190786

This Goat Polyclonal antibody specifically detects ILF2 in WB and ELISA. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for ILF2 antibody (Internal Region) (ABIN190786)

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ILF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ILF2 Antibody

Binding Specificity: Internal Region

Purpose: ILF2 / NF45

Sequence: EKAYEKPPEK KEG

Specificity: This antibody is expected to recognize both reported isoforms (NP_004506.2, NP_001254738.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKAYEKPPEKKEG, from the internal region (near the C Terminus) of the protein sequence according to NP_004506.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 43 kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1 kDa according to NP_004506.2). Recommended concentration: 0.03-0.1 μg/mL. This antibody has been successfully used in WB in the following papers: Ye J et al

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507). This data is from a previous batch, not on sale.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ILF2 antibody (ABIN190786)

Shamanna, Hoque, Peery, Mathews: "Induction of p53, p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells." in: Oncogene, Vol. 32, Issue 43, pp. 5176-85, (2013) (PubMed).

Shamanna, Hoque, Lewis-Antes, Azzam, Lagunoff, Peery, Mathews: "The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining." in: Molecular and cellular biology, Vol. 31, Issue 23, pp. 4832-43, (2011) (PubMed).

Target Details for ILF2

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Alternative Name: ILF2

Background: ILF2, NF45, interleukin enhancer binding factor 2, 45 kDa , MGC8391, PRO3063 , interleukin enhancer binding factor 2, nuclear factor of activated T-cells, 45- kDa

Gene ID: 3608, 67781, 310612

NCBI Accession: NP_004506, NP_001254738