IFIH1 antibody (Internal Region)

Catalog No. ABIN190791

The Goat Polyclonal anti-IFIH1 antibody (ABIN190791) specifically detects IFIH1 in ELISA. The antibody is reactive with Mouse samples.

Quick Overview for IFIH1 antibody (Internal Region) (ABIN190791)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IFIH1 antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-IFIH1 Antibody

Binding Specificity: Internal Region

Purpose: MDA5 / Ifih1 (mouse)

Sequence: KQKLRNNDLK KQN

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KQKLRNNDLKKQN, from the internal region of the protein sequence according to NP_082111.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 80 kDa band in Mouse Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116 kDa

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Alternative Name: Ifih1

Background: Ifih1, interferon induced with helicase C domain 1, 9130009C22Rik, Helicard, Hlcd, MDA-5, MDA5, MGC90959, melanoma differentiation associated protein-5

Gene ID: 71586

NCBI Accession: NP_082111

Pathways: Activation of Innate immune Response