PAX6 antibody (Internal Region)

Catalog No. ABIN190794

This anti-PAX6 antibody is a Goat Polyclonal antibody detecting PAX6 in WB, ELISA and FACS. Suitable for Human and Mouse.

Quick Overview for PAX6 antibody (Internal Region) (ABIN190794)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PAX6 Antibody

Binding Specificity: Internal Region

Purpose: PAX6 (internal)

Sequence: REEKLRNQRR QASN

Specificity: This antibody is expected to recognize all reported isoforms: NP_000271.1, NP_001595.2, NP_001297088.1, NP_001355839.1, NP_001355840.1, NP_001355841.1, NP_001355844.1, NP_001355847.1, NP_001355849.1, NP_001355850.1, NP_001355851.1, NP_001355857.1, NP_0013

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-REEKLRNQRRQASN, from the internal region of the protein sequence according to NP_000271.1, NP_001595.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26 kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8 kDa according to Human NP_001

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: PAX6

Background: PAX6, paired box gene 6 (aniridia, keratitis), AN, AN2, D11S812E, MGC17209, MGDA, WAGR, paired box gene 6, paired box homeotic gene 6 (aniridia, keratitis), paired box homeotic gene-6

Gene ID: 5080, 18508, 25509

NCBI Accession: NP_000271, NP_001595

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis