Acetyl-CoA Carboxylase beta antibody (ACACB) (Internal Region)

Catalog No. ABIN190803

Product Details anti-Acetyl-CoA Carboxylase beta Antibody

Target:

Acetyl-CoA Carboxylase beta (ACACB)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase beta antibody is un-conjugated

Application: ELISA, Enzyme Immunoassay (EIA)

Purpose: ACACB

Sequence: QLGEPDLSDK DRKD

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QLGEPDLSDKDRKD, from the internal region of the protein sequence according to NP_001084.2.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Preliminary experiments gave an approx 28 kDa band in Human Heart lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 277 kDa according to NP_001084.2. The 28 kDa band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ACACB captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Acetyl-CoA Carboxylase beta

Target:

Acetyl-CoA Carboxylase beta (ACACB)

Alternative Name: ACACB (ACACB Antibody Abstract)

Synonyms: F15C21.2, F15C21_2, acetyl-CoA carboxylase 2, ACC2, ACCB, HACC275, Acc2, AI597064, AW743042, Accb, acetyl-CoA carboxylase 2, acetyl-CoA carboxylase beta, acetyl-Coenzyme A carboxylase beta, ACC2, ACACB, Acacb

Background: ACACB, acetyl-Coenzyme A carboxylase beta , ACCB, HACC275 , acetyl-CoA carboxylase 2

Gene ID: 32

NCBI Accession: NP_001084

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process