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DS WB Results: Preliminary experiments gave an approx 28 kDa band in Human Heart lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 277 kDa according to NP_001084.2. The 28 kDa band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?
Peptide ELISA: antibody detection limit dilution 1:32000.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ACACB captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml