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Acetyl-CoA Carboxylase beta antibody (ACACB) (Internal Region) Primary Antibody

ACACB Reactivity: Human ELISA, EIA Host: Goat Polyclonal unconjugated
Catalog No. ABIN190803
$481.84
Plus shipping costs $45.00
100 μg
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  • Target
    Binding Specificity
    Internal Region
    Reactivity
    Human
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    This Acetyl-CoA Carboxylase beta antibody is un-conjugated
    Application
    ELISA, Enzyme Immunoassay (EIA)
    Purpose
    ACACB
    Sequence
    QLGEPDLSDK DRKD
    Predicted Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QLGEPDLSDKDRKD, from the internal region of the protein sequence according to NP_001084.2.
    Isotype
    IgG
  • Application Notes

    DS WB Results: Preliminary experiments gave an approx 28 kDa band in Human Heart lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 277 kDa according to NP_001084.2. The 28 kDa band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ACACB captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Alternative Name
    ACACB (ACACB Antibody Abstract)
    Synonyms
    F15C21.2, F15C21_2, acetyl-CoA carboxylase 2, ACC2, ACCB, HACC275, Acc2, AI597064, AW743042, Accb, acetyl-CoA carboxylase 2, acetyl-CoA carboxylase beta, acetyl-Coenzyme A carboxylase beta, ACC2, ACACB, Acacb
    Background
    ACACB, acetyl-Coenzyme A carboxylase beta , ACCB, HACC275 , acetyl-CoA carboxylase 2
    Gene ID
    32
    NCBI Accession
    NP_001084
    Pathways
    AMPK Signaling, Ribonucleoside Biosynthetic Process
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