GPX3 antibody (Internal Region)

Catalog No. ABIN190837

This Goat Polyclonal antibody specifically detects GPX3 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for GPX3 antibody (Internal Region) (ABIN190837)

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GPX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-GPX3 Antibody

Binding Specificity: Internal Region

Purpose: GPX3

Sequence: NQFGKQEPGE NSE

Specificity: This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NQFGKQEPGENSE, from the internal region of the protein sequence according to NP_002075.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 26 kDa band observed in Mouse Heart lysates (calculated MW of 27.7 kDa according to Mouse NP_001077398.1 [isoform1] and 25.4 kDa according to Mouse NP_032187.2 [isoform 2] ). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hou

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GPX3

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Alternative Name: GPX3

Background: GPX3, glutathione peroxidase 3 (plasma), GPx-P, GSHPx-3, GSHPx-P , extracellular glutathione peroxidase, plasma glutathione peroxidase 3

Gene ID: 2878, 14778, 64317

NCBI Accession: NP_002075

Pathways: Thyroid Hormone Synthesis