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PIAS2 antibody (Internal Region)

This Goat Polyclonal antibody specifically detects PIAS2 in ELISA and IHC. It exhibits reactivity toward Human.
Catalog No. ABIN190909

Quick Overview for PIAS2 antibody (Internal Region) (ABIN190909)

Target

See all PIAS2 Antibodies
PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Reactivity

  • 59
  • 27
  • 23
  • 10
  • 8
  • 8
  • 8
  • 5
  • 4
  • 1
  • 1
  • 1
Human

Host

  • 53
  • 6
  • 1
  • 1
Goat

Clonality

  • 55
  • 6
Polyclonal

Conjugate

  • 45
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PIAS2 antibody is un-conjugated

Application

  • 49
  • 22
  • 13
  • 8
  • 6
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC)

Grade

Verified
  • Binding Specificity

    • 10
    • 10
    • 9
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Purpose

    PIAS2

    Sequence

    KEAMKVSSQP CTKIE

    Specificity

    This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.

    Cross-Reactivity

    Dog, Human, Mouse, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence KEAMKVSSQPCTKIE, from the internal region of the protein sequence according to NP_775298.1, NP_004662.2.

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5 μg/mL.

    Western Blot: Preliminary experiments gave an approx 90 kDa band in lysates of cell line A431after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

    Alternative Name

    PIAS2

    Background

    PIAS2, protein inhibitor of activated STAT, 2 , MGC102682, MIZ1, PIASX-ALPHA, PIASX-BETA, SIZ2, miz , Msx-interacting-zinc finger, protein inhibitor of activated STAT X, ARIP3

    Gene ID

    9063, 17344

    NCBI Accession

    NP_775298, NP_004662

    Pathways

    JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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