PIAS2 antibody (Internal Region)

Catalog No. ABIN190909

This Goat Polyclonal antibody specifically detects PIAS2 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for PIAS2 antibody (Internal Region) (ABIN190909)

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PIAS2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PIAS2 Antibody

Binding Specificity: Internal Region

Purpose: PIAS2

Sequence: KEAMKVSSQP CTKIE

Specificity: This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KEAMKVSSQPCTKIE, from the internal region of the protein sequence according to NP_775298.1, NP_004662.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5 μg/mL.

Western Blot: Preliminary experiments gave an approx 90 kDa band in lysates of cell line A431after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Alternative Name: PIAS2

Background: PIAS2, protein inhibitor of activated STAT, 2 , MGC102682, MIZ1, PIASX-ALPHA, PIASX-BETA, SIZ2, miz , Msx-interacting-zinc finger, protein inhibitor of activated STAT X, ARIP3

Gene ID: 9063, 17344

NCBI Accession: NP_775298, NP_004662

Pathways: JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling