LEF1 antibody (AA 349-399)

Catalog No. ABIN1909578

The Rabbit Polyclonal anti-LEF1 antibody (ABIN1909578) specifically detects LEF1 in WB, IHC, IF, IP and IHC (p). The antibody is reactive with Human, Goat and Chicken samples.

Quick Overview for LEF1 antibody (AA 349-399) (ABIN1909578)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human, Goat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LEF1 Antibody

Binding Specificity: AA 349-399

Specificity: Region between residue 349 and 399 of human Lymphoid Enhancer-Binding Factor 1 using the numbering given in entry NP_057353.1 (GeneID 51176).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:250 - 1:1000), IHC, IHC-P (1:500 - 1:2000), IP (2 - 10 μg/mg lysate), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background: Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA

Gene ID: 51176

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding