MED12 antibody (C-Term)

Catalog No. ABIN1912853

The Rabbit Polyclonal anti-MED12 antibody is suitable to detect MED12 in samples from Human, Mouse, Rat, Cow, Dog and Pig. It has been validated for IHC, WB, IHC (p), IP and IF.

Quick Overview for MED12 antibody (C-Term) (ABIN1912853)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED12 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-MED12 Antibody

Binding Specificity: AA 2150-2212, C-Term

Specificity: Region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 using the numbering given in entry NP_005111.1 (GeneID 9968).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:250 - 1:1000), IHC, IHC-P (1:500 - 1:2000), IP (2 - 5 μg/mg lysate), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Ovarian Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Alternative Name: MED12

Background: Name/Gene ID: MED12

Synonyms: MED12, ARC240, CAG repeat protein 45, CAGH45, FG syndrome 1, Hypothetical protein kiaa0192, FGS1, Mediator complex subunit 12, Putative mediator subunit 12, MED12S, TNRC11, TRAP230, HOPA, Human opposite paired, KIAA0192, OKS, OPA-containing protein

Gene ID: 9968

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation