MED12 antibody (AA 580-660)

Catalog No. ABIN7226190

This Rabbit Polyclonal antibody specifically detects MED12 in ELISA, IHC, WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for MED12 antibody (AA 580-660) (ABIN7226190)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED12 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-MED12 Antibody

Binding Specificity: AA 580-660

Purpose: Rabbit Anti-TRAP230 Polyclonal Antibody

Specificity: TRAP230 Polyclonal Antibody detects endogenous levels of TRAP230 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TRAP230 at AA range: 580-660

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Alternative Name: TRAP230

Background: MED12, ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230, Mediator of RNA polymerase II transcription subunit 12, Activator-recruited cofactor 240 kDa component, ARC240, CAG repeat protein 45, Mediator complex subunit 12, OPA-containing proteThe initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1. MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by the gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

Gene ID: 9968

UniProt: Q93074

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation