IBSP antibody

Catalog No. ABIN191813

This Chicken Polyclonal antibody specifically detects IBSP in WB, IHC (p), EIA, IF, RIA and FACS. It exhibits reactivity toward Human.

Quick Overview for IBSP antibody (ABIN191813)

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This IBSP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Radioimmunoassay (RIA), Flow Cytometry (FACS)

Product Details anti-IBSP Antibody

Specificity: This antibody detects Bone Sialoprotein. There were no cross reactivities obtained with Human Osteonectin, Human Osteopontin and Human Alkaline Phosphatase.

Purification: Purified

Immunogen: Human Bone Sialoprotein (bone extract)

Application Details

Application Notes: ELISA (1 μg/mL). RIA (20 ng/mL). Flow Cytometry. Western blot (2 ng/mL). Immunohistochemistry on Paraffin Sections (2 ng/mL). Immunocytochemistry (1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in aqua bidest to initial volume.

Buffer: PBS, pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at -20 °C.

Target Details for IBSP

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Alternative Name: Bone Sialoprotein 2

Background: The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12 % of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.Synonyms: BNSP, BSP II, Bone sialoprotein II, Cell-binding sialoprotein, IBSP, Integrin-binding sialoprotein

Gene ID: 3381

NCBI Accession: NP_004958

UniProt: P21815