Patched 1 antibody (Cytoplasmic Domain)

Catalog No. ABIN1927748

The Rabbit Polyclonal anti-Patched 1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Patched 1 in samples from Human and Mouse.

Quick Overview for Patched 1 antibody (Cytoplasmic Domain) (ABIN1927748)

Target: Patched 1 (PTCH1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Patched 1 Antibody

Binding Specificity: Cytoplasmic Domain

Brand: IHC-plus™

Specificity: Recognizes human Protein patched homolog 1 at ~160kD. Species cross-reactivity: mouse.

Purification: Affinity purified

Immunogen: Linear peptide corresponding to the cytoplasmic domain of human Protein patched homolog 1 (KLH).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.1 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 0.1 μg/mL detected Patched on 10 μg of human skeletal muscle, fetal tissue lysate. Heat induced epitope retreival with citrate buffer,  pH 6.0 is recommended when using in immunohistochemistry.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1 / Patched

Background: Name/Gene ID: PTCH1

Synonyms: PTCH1, NBCCS, Patched 1, Patched homolog 1, Protein patched homolog 1, PTC1, PTCH, PTCH protein +12b, PTCH protein -10, PTCH protein -3,4,5, Patched, Patched protein homolog 1, BCNS, HPE7, Patched homolog, PTC, PTCH protein +4, PTCH11

Gene ID: 5727

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation