14-3-3 sigma/SFN antibody (AA 198-248)

Catalog No. ABIN1934177

The Rabbit Polyclonal anti-14-3-3 sigma/SFN antibody has been validated for WB, IHC, IF, IP, IHC (p) and PLA. It is suitable to detect 14-3-3 sigma/SFN in samples from Human.

Quick Overview for 14-3-3 sigma/SFN antibody (AA 198-248) (ABIN1934177)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: AA 198-248

Specificity: Region between residue 198 and 248 of human 14-3-3 sigma using the numbering given in entry NP_006133.1 (GeneID 2810).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:500 - 1:2500), IHC, IHC-P (1:1000 - 1:5000), IP (2 - 5 μg/mg lysate), PLA (1:1000 - 1:10000), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN / Stratifin / 14-3-3 Sigma

Background: Name/Gene ID: SFN

Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS

Gene ID: 2810

Pathways: p53 Signaling, Myometrial Relaxation and Contraction