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p53 antibody (AA 50-100)

This anti-p53 antibody is a Rabbit Polyclonal antibody detecting p53 in WB, IHC, IF, FACS, IP, IHC (p) and PLA. Suitable for Human.
Catalog No. ABIN1943403

Quick Overview for p53 antibody (AA 50-100) (ABIN1943403)

Target

See all p53 (TP53) Antibodies
p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This p53 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)
  • Binding Specificity

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    AA 50-100

    Specificity

    Region between residues 50 and 100 of human tumor protein p53 using the numbering given in SwissProt entry P04637 (GeneID 7157).

    Purification

    Immunoaffinity purified

    Isotype

    IgG
  • Application Notes

    Approved: Flo, IF (1:500 - 1:2500), IHC, IHC-P (1:1000 - 1:5000), IP (2 - 10 μg/mg lysate), PLA (1:1000 - 1:20000), WB (1:5000 - 1:25000)

    Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Lung Adenocarcinoma, Skin Squamous Cell Carcinoma.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C for up to 1 year.

    Expiry Date

    12 months
  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Alternative Name

    TP53 / p53

    Background

    Name/Gene ID: TP53
    Family: P53

    Synonyms: TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1

    Gene ID

    7157

    Pathways

    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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