NCF1 antibody

Catalog No. ABIN1944805

This Rabbit Polyclonal antibody specifically detects NCF1 in WB and IF. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for NCF1 antibody (ABIN1944805)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NCF1 Antibody

Isotype: IgG1

Application Details

Application Notes: IF: 1:100. WB: 1:250-1:1000. WB: 1:250-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-NCF1 antibody (ABIN1944805)

Görlach, Lee, Roesler, Hopkins, Christensen, Green, Chanock, Curnutte: "A p47-phox pseudogene carries the most common mutation causing p47-phox- deficient chronic granulomatous disease." in: The Journal of clinical investigation, Vol. 100, Issue 8, pp. 1907-18, (1997) (PubMed).

Rodaway, Teahan, Casimir, Segal, Bentley: "Characterization of the 47-kilodalton autosomal chronic granulomatous disease protein: tissue-specific expression and transcriptional control by retinoic acid." in: Molecular and cellular biology, Vol. 10, Issue 10, pp. 5388-96, (1990) (PubMed).

Volpp, Nauseef, Donelson, Moser, Clark: "Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 18, pp. 7195-9, (1989) (PubMed).

Lomax, Leto, Nunoi, Gallin, Malech: "Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease." in: Science (New York, N.Y.), Vol. 245, Issue 4916, pp. 409-12, (1989) (PubMed).

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: p47 phox

Background: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Molecular Weight: 44652 Da

Gene ID: 653361

UniProt: P14598

Pathways: PI3K-Akt Signaling