MEK1 antibody (pThr286)

Catalog No. ABIN1959461

The Rabbit Polyclonal anti-MEK1 antibody is suitable to detect MEK1 in samples from Human, Mouse, Rat, Cow, Rabbit, Pig and Horse. It has been validated for IHC, IF, IHC (p), IP and PLA.

Quick Overview for MEK1 antibody (pThr286) (ABIN1959461)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Product Details anti-MEK1 Antibody

Binding Specificity: pThr286

Specificity: Region of human MAPK/ERK kinase 1 (dual specificity mitogen-activated protein kinase kinase 1) surrounding threonine 286 using the numbering given in entry NP_002746.1 (Gene ID 5604) when the threonine residue is phosphorylated.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:500 - 1:2500), IHC, IHC-P (1:1000 - 1:5000), IP (2 - 10 μg/mg lysate), PLA (1:1000 - 1:20000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Lung Cancer, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1 / MEK1

Background: Name/Gene ID: MAP2K1
Subfamily: MAP2K
Family: Protein Kinase

Synonyms: MAP2K1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MKK1, PRKMK1, MEK 1, MAP kinase kinase 1, MAPKK1, MEK1

Gene ID: 5604

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling