Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Myc (phospho-Thr58) antibody detects endogenous levels of Myc only when phosphorylated at threonine 58.
The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of threonine 58 (L-P-TP-P-P).
Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
For Research Use only
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.