CD14 antibody

Catalog No. ABIN1967146

This Mouse Monoclonal antibody specifically detects CD14 in FACS, WB, IP, ELISA, Func, BR and Neut. It exhibits reactivity toward Human and Primate.

Quick Overview for CD14 antibody (ABIN1967146)

Target: CD14

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA, Functional Studies (Func), Blocking Reagent (BR), Neutralization (Neut)

Clone: MEM-18

Product Details anti-CD14 Antibody

Specificity: Monoclonal antibody MEM-18 reacts with CD14, a 53-55 kD GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: Crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

Type of Immunogen: Protein extract

Isotype: IgG1

Application Details

Application Notes: Approved: Block, ELISA, Flo, Func, IP, Neut, WB

Usage: Flow Cytometry: Recommended dilution - 4 μg/mL. Western Blotting: Application note - Non-reducing conditions. ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, sterile filtered.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: Name/Gene ID: CD14

Synonyms: CD14, CD14 Molecule, CD14 antigen

Gene ID: 929

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades