p53 antibody (pSer315)
Quick Overview for p53 antibody (pSer315) (ABIN196788)
Target
See all p53 (TP53) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pSer315
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Specificity
- This antibody detects endogenous levels of p53 only when phosphorylated at Serine 315.
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Purification
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogen
- The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of Serine 315 (S-S-SP-P-Q).
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anti-Tumor Protein P53 (TP53) (AA 1-100) antibody
TP53 Reactivity: Human WB, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer392) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 Reactivity: Human WB, IF, IHC (p), IP, ICC, ChIP Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 Reactivity: Human WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 Reactivity: Human, Mouse, Rat, Goat WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer20) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer15) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (acLys319) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 293-322) antibodyTP53 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB7777 unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 Reactivity: Human WB, IHC, IP Host: Rabbit Monoclonal unconjugated
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Application Notes
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Store the antibody (in aliquots) at -20 °C.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Alternative Name
- p53 (TP53)
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Background
- Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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Gene ID
- 7157
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NCBI Accession
- NP_000537
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UniProt
- P04637
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Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
Target
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