NCOA1 antibody (pTyr529)
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- Target See all NCOA1 Antibodies
- NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))
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Binding Specificity
- pTyr529
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NCOA1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- The antibody detects endogenous levels of Src only when phosphorylated at Tyrosine 529.
- Purification
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).
- Top Product
- Discover our top product NCOA1 Primary Antibody
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anti-Nuclear Receptor Coactivator 1 (NCOA1) (AA 1222-1441) antibody
NCOA1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) (AA 1338-1367), (C-Term) antibodyNCOA1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB14368 unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) (AA 1338-1367) antibodyNCOA1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) (AA 284-393) antibodyNCOA1 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) (AA 201-300) antibodyNCOA1 Reactivity: Mouse WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro), ICC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) antibodyNCOA1 Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) (AA 186-214), (N-Term) antibodyNCOA1 Reactivity: Human WB Host: Rabbit Polyclonal RB14365 unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) (AA 594-625) antibodyNCOA1 Reactivity: Human WB Host: Rabbit Polyclonal RB14285 unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) antibodyNCOA1 Reactivity: Human, Mouse IF Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Coactivator 1 (NCOA1) (AA 611-660) antibodyNCOA1 Reactivity: Human, Rat, Dog, Monkey WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody (in aliquots) at -20 °C.
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- Target
- NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))
- Alternative Name
- SRC1 (NCOA1 Products)
- Synonyms
- F-SRC-1 antibody, KAT13A antibody, RIP160 antibody, SRC1 antibody, bHLHe42 antibody, bHLHe74 antibody, si:dkey-5g7.6 antibody, si:dkey-34f16.6 antibody, NCOA1 antibody, SRC-1 antibody, nuclear receptor coactivator 1 antibody, NCOA1 antibody, ncoa1 antibody, Ncoa1 antibody
- Background
- Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src
- Gene ID
- 6714
- NCBI Accession
- NP_005408
- UniProt
- P12931
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
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