STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pSer727)

Details for Product anti-STAT1 Antibody No. ABIN196892
Antigen
  • CANDF7
  • ISGF-3
  • STAT91
  • 2010005J02Rik
  • AA408197
  • XStat1
  • STAT4
  • DD6G4-4
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • stat1
  • signal transducer and activator of transcription 1
  • signal transducer and activator of transcription 1 L homeolog
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription 4
  • signal transducer and activator of transcription 1a
  • STAT1
  • Stat1
  • stat1.L
  • stat4
  • stat1a
Epitope
pSer727
97
71
50
17
16
15
9
8
8
8
8
7
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
554
309
278
33
29
26
22
22
16
13
11
7
7
5
2
2
2
1
1
1
1
1
Host
Rabbit
470
105
2
2
1
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
18
14
13
11
11
11
11
11
11
6
6
5
5
5
5
5
4
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
432
200
158
112
90
65
46
20
18
9
7
7
4
3
1
1
Options
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human STAT1 around the phosphorylation site of serine 727 (P-M-Sp-P-E).
Specificity This antibody detects endogenous levels of STAT1 only when phosphorylated at Serine 727.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Plasmids, Primers & others Plasmids, Primers & others STAT1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Gene ID 9606
UniProt P42224
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Image no. 1 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN196892) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (Image 1)
Image no. 2 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN196892) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (Image 2)
Image no. 3 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN196892) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (Image 3)
Background publications Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Yuan, Shioya, Ishiko, Sun, Gu, Huang, Lu, Kharbanda, Weichselbaum, Kufe: "p73 is regulated by tyrosine kinase c-Abl in the apoptotic response to DNA damage." in: Nature, Vol. 399, Issue 6738, pp. 814-7, 1999 (PubMed).

Quelle, Thierfelder, Witthuhn, Tang, Cohen, Ihle: "Phosphorylation and activation of the DNA binding activity of purified Stat1 by the Janus protein-tyrosine kinases and the epidermal growth factor receptor." in: The Journal of biological chemistry, Vol. 270, Issue 35, pp. 20775-80, 1995 (PubMed).

Schindler, Fu, Improta, Aebersold, Darnell: "Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7836-9, 1992 (PubMed).

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