STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pSer727)

Details for Product anti-STAT1 Antibody No. ABIN196892
Target
Binding Specificity
pSer727
50
37
29
17
15
15
8
8
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Rat
294
161
156
24
24
23
20
18
13
10
8
6
5
5
2
1
1
1
1
1
Host
Rabbit
238
63
2
2
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
15
11
11
10
9
9
9
8
8
5
5
4
4
4
4
4
3
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
208
96
88
60
55
48
23
22
11
8
4
4
4
1
1
Options
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human STAT1 around the phosphorylation site of serine 727 (P-M-Sp-P-E).
Specificity This antibody detects endogenous levels of STAT1 only when phosphorylated at Serine 727.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Target
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Gene ID 9606
UniProt P42224
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Images
Image no. 1 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN196892) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (Image 1)
Image no. 2 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN196892) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (Image 2)
Image no. 3 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN196892) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (Image 3)