PRKDC antibody (pThr2609)

Catalog No. ABIN196899

The Rabbit Polyclonal anti-PRKDC antibody is suitable to detect PRKDC in samples from Human. It has been validated for WB.

Quick Overview for PRKDC antibody (pThr2609) (ABIN196899)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRKDC Antibody

Binding Specificity: pThr2609

Specificity: DNA PKcs (phospho-Thr2609) antibody detects endogenous levels of DNA PKcs only when phosphorylated at threonine 2609.

Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human DNA PKcs around the phosphorylation site of threonine 2609 (V-E-TP-Q-A).

Application Details

Application Notes: Suitable for use in Suitable for use in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: DNA-PKcs / PRKDC / XRCC7

Background: DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460

Gene ID: 5591

NCBI Accession: NP_001075109

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response