Paxillin antibody (Tyr118)

Catalog No. ABIN197278

This Rabbit Polyclonal antibody specifically detects Paxillin in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Paxillin antibody (Tyr118) (ABIN197278)

Target: Paxillin (PXN)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Paxillin Antibody

Binding Specificity: Tyr118

Sequence: H-V-YP-S-F

Specificity: This antibody detects endogenous levels of total Paxillin protein.

Purification: Affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized non-phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118

Application Details

Application Notes: WB: 1: 500-1: 1000. IHC: 1: 50-1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin / PXN

Background: Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).Synonyms: FLJ16691

Gene ID: 5829

NCBI Accession: NP_001074324

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling