PKC mu antibody (Ser738)

Catalog No. ABIN197312

The Rabbit Polyclonal anti-PKC mu antibody has been validated for WB and IF. It is suitable to detect PKC mu in samples from Human, Mouse and Rat.

Quick Overview for PKC mu antibody (Ser738) (ABIN197312)

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC mu antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PKC mu Antibody

Binding Specificity: Ser738

Specificity: This antibody detects endogenous levels of total PKD/PKCμ protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human PKD/PKCmy around the phosphorylation site of serine 738 (E-K-Sp-F-R).

Application Details

Application Notes: Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PKC mu

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Alternative Name: PRKD1

Background: Protein Kinase C mu, is a ~140 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC mu (also known as Protein Kinase D or PKD) is implicated in the regulation of multiple cellular processes including Golgi organization and membrane transport in epithelial cells. PKC mu is phosphorylated on serine 742 (serine 748 for the mouse sequence) in the activation loop in a PKC-dependent pathway, mainly by PKC eta and PKC epsilon. This is critical for its catalytic activity, substrate phosphorylation and role in activating the ERK1 MAP Kinase signaling cascade.Synonyms: PKC D1, PKC mu, PKD, PKD1, PRKCM, Protein kinase C mu type, Protein kinase D, Serine/threonine-protein kinase D1, nPKC-D1, nPKC-mu

Gene ID: 5587

NCBI Accession: NP_002733

UniProt: Q15139

Pathways: Myometrial Relaxation and Contraction