Merlin antibody (AA 516-520)

Catalog No. ABIN197520

This anti-Merlin antibody is a Rabbit Polyclonal antibody detecting Merlin in WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Merlin antibody (AA 516-520) (ABIN197520)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Merlin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Merlin Antibody

Binding Specificity: AA 516-520

Specificity: This antibody detects endogenous levels of total Merlin protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around aa 516~520 (R-L-S-M-E) derived from Human Merlin

Application Details

Application Notes: Western Blot: 1/500approx. 1/1000. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Alternative Name: Merlin

Background: Neurofibromin 2 (NF2) is a tumor suppressor gene encoding the protein Merlin. Merlin is closely related to the ERM (ezrin, radixin, moesin) family of proteins. The precise funtion of Merlin is not clear. It is thought to provide a link between the actin cytoskeleten and membrane associated proteins, playing a role in transduction of extracellular signals. It has been implicated in cell proliferation and cellular motility. Mutations in the NF2 gene cause neurofibromatosis type II, a condition characterised by the development of tumors in the central nervous system.Synonyms: Moesin-ezrin-radixin-like protein, NF2, Neurofibromin-2, SCH, Schwannomerlin, Schwannomerlin, Schwannomin, Schwannomin

Gene ID: 4771

NCBI Accession: NP_000259

UniProt: P35240

Pathways: Cell-Cell Junction Organization