Merlin antibody (pSer10)

Catalog No. ABIN1532019

Cited in 1 publication. The Rabbit Polyclonal anti-Merlin antibody (ABIN1532019) specifically detects Merlin in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Merlin antibody (pSer10) (ABIN1532019)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Merlin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Merlin Antibody

Binding Specificity: AA 1-50, pSer10

Purpose: NF2 (phospho Ser10) Antibody

Specificity: Phospho-NF2 (S10) Polyclonal Antibody detects endogenous levels of NF2 protein only when phosphorylated at S10.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Merlin around the phosphorylation site of Ser10. AA range:1-50

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-Merlin antibody (ABIN1532019)

Yu, Zeidel, Hill: "Cellular expression profile for interstitial cells of cajal in bladder - a cell often misidentified as myocyte or myofibroblast." in: PLoS ONE, Vol. 7, Issue 11, pp. e48897, (2012) (PubMed).

Target Details for Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Alternative Name: Merlin

Background:

Synonyms: NF2, SCH, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin

Background: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development, in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008],

Gene Name: NF2

Protein Name: Merlin

Molecular Weight: 69 kDa

Gene ID: 4771

OMIM: 101000

UniProt: P35240

Pathways: Cell-Cell Junction Organization