BRCA1 antibody (AA 250-300)

Catalog No. ABIN1977065

The Rabbit Polyclonal anti-BRCA1 antibody has been validated for WB, IHC, IF, IHC (p), IP and PLA. It is suitable to detect BRCA1 in samples from Human.

Quick Overview for BRCA1 antibody (AA 250-300) (ABIN1977065)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 250-300

Specificity: Region between residue 250 and 300 of human breast cancer 1 using the numbering given in entry NP_009225.1 (GeneID 672).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:250 - 1:2000), IHC, IHC-P (1:500 - 1:2000), IP (2 - 10 μg/mg lysate), PLA (1:1000 - 1:20000), WB (1:500 - 1:2500)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Ovarian Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background: Name/Gene ID: BRCA1

Synonyms: BRCA1, BROVCA1, BRCC1, Breast cancer 1, early onset, PPP1R53, PSCP, PNCA4, RING finger protein 53, RNF53, BRCAI, Breast cancer 1 early onset, IRIS

Gene ID: 672

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus